carolyn bertozzi biography

The increase of cell surface sialic acid is a characteristic shared by many tumor types. Driessen, M. D., Going, C. C., Woo, C. M., Pitteri, S. J., Bertozzi, C. R. New therapeutic avenues for NGLY1 deficiency, A TENSION-MEDIATED GLYCOCALYX FEEDBACK LOOP PROMOTES A MESENCHYMAL, STEM-LIKE PHENOTYPE IN GLIOBLASTOMA. 56Carolyn Bertozzi 12 Bertozzi won the prize for studying the sugar coats of cells. Sialic acid is a component of many tumor-associated oligosaccharide antigens. Parak, W. J., Gerion, D., Zanchet, D., Woerz, A. S., Pellegrino, T., Micheel, C., Williams, S. C., Seitz, M., Bruehl, R. E., Bryant, Z., Bustamante, C., Bertozzi, C. R., Alivisatos, A. P. Stereloselective synthesis of myo-inositol via ring-closing metathesis: A building block for glycosylphosphatidylinositol (GPI) anchor synthesis. Synthetic oligosaccharides and glycoconjugates provide materials for correlating structure with function. We found that the fucose salvage pathway enzymes are expressed during zebrafish embryogenesis but that they process the azide-modified substrates inefficiently. Nrf1 is constitutively translocated into the ER lumen, N-glycosylated, and then targeted for proteasomal degradation via the ER-associated degradation (ERAD) pathway. A library of potential bisubstrate analogue inhibitors (1) targeting sulfotransferase enzymes was generated by the chemoselective ligation of the PAPS mimic 2 with a panel of 447 aldehydes. In a prototypical experiment, a unique chemical motif, often as small as a single functional group, is incorporated into the target biomolecule using the cell's own biosynthetic machinery. These findings extend the known functions of sulfated molecules as general modulators of cell-cell interactions to include those between a bacterium and a human host. Exact mass measurements showed that S881 is highly unsaturated, tandem mass spectrometry indicated a polyisoprene-derived structure, and characterization of synthetic structural analogs confirmed that S881 is a previously undescribed sulfated derivative of dihydromenaquinone-9, the primary quinol electron carrier in M. tuberculosis. Our results suggest a correlation between decreased alkyne bond angle and increased cyclooctyne reactivity. Mass spectrometry assays allowed us to identify other acceptors, mainly integrins. Unlike existing systems for controlling gene expression in Mtb, the riboswitch does not require the co-expression of any accessory proteins: all of the regulatory machinery is encoded by a short DNA segment directly upstream of the target gene. Additionally, we demonstrate that in C. glutamicum, the peripheral peptidoglycan layer at the septal junction remains contiguous throughout septation, forming a diffusion barrier for the fluid mycomembrane. Lim, H. C., Sher, J. W., Rodriguez-Rivera, F. P., Fumeaux, C. n., Bertozzi, C. R., Bernhardt, T. G. The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. We functionalized cells with short oligonucleotides to impart specific adhesive properties. Converse, S. E., Mougous, J. D., Leavell, M. D., Leary, J. She also later developed a click reaction called the strain-promoted alkyne-azide cycloaddition, which, unlike other click reactions involving azides and alkynes, did not require toxic copper ions for the azide to snap together with the alkyne and was highly effective for tracking glycans in live cells. Here we explored their effect on corneal surface glycosylation using a metabolic label, tetra-acetylated N-azidoacetylgalactosamine (Ac4GalNAz). However, currently available agonists are heterogeneous glycoconjugates and polysaccharides from natural sources. PC-3 cells and primary human prostate cancer tissue were treated with peracetylated N-azidoacetylgalactosamine, resulting in metabolic labeling of cell surface glycans with the azidosugar. The data presented here was obtained with the application of a bioorthogonal chemical reporter strategy analyzing differential glycoprotein expression following the knock-down (KD) of the GALNT3 gene in the epithelial ovarian cancer (EOC) cell line A2780s. Many cellular activities are controlled by post-translational modifications, the study of which is hampered by the lack of specific reagents due in large part to their ubiquitous and non-immunogenic nature. Shieh, P., Hangauer, M. J., Bertozzi, C. R. Probing the Mycobacterial Trehalome with Bioorthogonal Chemistry. Generated in nine steps from a glucose analogue, DIMAC reacted with azide-labeled proteins and cells similarly to cyclooctynes. Recent work has implicated tyrosine sulfate as a determinant of protein-protein interactions involved in leukocyte adhesion, hemostasis and chemokine signaling. Dai, T. n., Xie, J. n., Zhu, Q. n., Kamariza, M. n., Jiang, K. n., Bertozzi, C. R., Rao, J. n. Optimal Dissociation Methods Differ for N- and O-glycopeptides. By mutating catalytic residues of two such enzymes, we engineered mucin-selective binding agents with retained glycoform preferences. View details for Web of Science ID 000232605600062. This type of enzyme catalyzes the initial step of mucin-type O-glycosylation, that is, the transfer of GalNAc in O-glycosidic linkage to serine and threonine residues in polypeptides. Hatzios, S. K., Schelle, M. W., Newton, G. L., Sogi, K. M., Holsclaw, C. M., Fahey, R. C., Bertozzi, C. R. Isotopic Signature Transfer and Mass Pattern Prediction (IsoStamp): An Enabling Technique for Chemically-Directed Proteomics. The lack of a defined consensus sequence for the ppGalNAcTs makes the prediction of mucin-type O-linked glycosylation difficult based on primary sequence alone. Inhibitors of a key step of O-linked glycan biosynthesis can be discovered from a directed library screen. A revised, highly practical synthesis of the precursor N(alpha)-Fmoc-Thr(Ac(3)-alpha-D-GalNAc) allowed us to produce sufficient quantities of the glycopeptide for mechanistic assays. View details for Web of Science ID 000296312200014. Exhaled air and bioaerosol volumes were estimated using continuous CO2 monitoring and airborne particle counting, respectively. We report the synthesis of an alpha-formylglycine building block suitable for Fmoc-based solid-phase peptide synthesis. Liposomes displaying 3'-sulfo Lewis(X)-like oligosaccharides, on the other hand, show slight loss of binding with introduction of additional anionic functional groups for E- and P-selectin and negligible change for L-selectin. Glycoproteins are typically expressed as mixtures of glycoforms, their oligosaccharides being generated by a template-independent biosynthetic process. A panel of analogues with ketone-containing N-acyl groups that varied in the length or steric bulk was chemically synthesized and tested for metabolic conversion to cell surface glycans. Cyclodextrin complexation is therefore a promising approach for stabilizing compounds that possess the high intrinsic reactivities desired for Cu-free click chemistry. Accordingly, treatment of cells with N-azidoacetylglucosamine results in the metabolic incorporation of the azido sugar into nuclear and cytoplasmic proteins. Protein-based assemblies with ordered nanometer-scale features in three dimensions are of interest as functional nanomaterials but are difficult to generate. Poly-alpha2,8-sialic acid (PSA) has been implicated in numerous normal and pathological processes, including development, neuronal plasticity, and tumor metastasis. A., Bertozzi, C. R., Marahiel, M. A., Burkart, M. D. Uridine-Based Inhibitors as New Leads for Antibiotics Targeting Escherichia coli LpxC. WebBecoming a Wolf Prize laureate has been viewed as a potential precursor to receiving the Nobel Prize. Several important human pathogens are represented in the Corynebacterineae suborder, including Mycobacterium tuberculosis and Corynebacterium diphtheriae. [19][39] Redwood Bioscience was acquired by Catalent Pharma Solutions in 2014. View details for Web of Science ID 000222420300014. Selective chemical reactions that are orthogonal to the diverse functionality of biological systems have become important tools in the field of chemical biology. [reaction: see text] Nearly all known sulfatases share a common active site modification that is required for their activity: conversion of cysteine to alpha-formylglycine. Photoacoustic calorimetry combined with established absorption and fluorescence methodologies provides a complete arsenal for characterizing the photophysical properties of many systems. Our method imparts N-glycopeptide mass envelopes with a pattern that is computationally distinguishable from background ions. View details for Web of Science ID 000172181700022. See vol 283, pg 4460, 2008). Using a high-throughput enzyme-linked lectin assay (ELLA), two inhibitors of murine ppGalNAcT-1 (K(I) approximately 8 microM) were identified that also inhibit several other members of the family. This position statement originated from a working group meeting convened on April 15, 2015, by the NHLBI and incorporates follow-up contributions by the participants as well as other thought leaders subsequently consulted, who together represent research fields relevant to all branches of the NIH. The phosphine-luciferin probe is therefore poised for many applications in real-time imaging in cells and whole animals. She described the reaction between the modified sugar and the fluorescent molecule as bioorthogonal. Presented herein is the synthesis and evaluation of a bisubstrate analogue designed to inhibit estrogen sulfotransferase. The ligation exploits the bioorthogonal reaction of aldehydes and alkoxyamines to form an intermediate oxyiminium ion; this intermediate undergoes intramolecular C-C bond formation with an indole nucleophile to form an oxacarboline product that is hydrolytically stable. The apparent rate constants for the hydrolysis and disappearance of the cell surface conjugates were determined, as well as the apparent rate constant for the formation of covalent bonds with cell surface ketones. A broader theme that emerged was the urgent need to bring the glycosciences back into the mainstream of biology by integrating relevant education into the curricula of medical, graduate, and postgraduate training programs, thus generating a critical sustainable workforce that can advance the much-needed translation of glycosciences into a more complete understanding of biology and the enhanced practice of medicine. Here, we discuss the transcriptional and biochemical mechanisms of sulfur metabolism regulation in Mtb and potential small molecule regulators of the sulfate assimilation pathway that are collectively poised to aid this intracellular pathogen in its expert manipulation of the host. Here we show that MmpL8, a member of a large family of predicted lipid transporters in M. tuberculosis, is required for SL-1 production. View details for DOI 10.1073/pnas.1222041110, View details for Web of Science ID 000322771100029, View details for PubMedCentralID PMC3740907. Despite recent progress, important mechanistic questions, such as the precise bacterial targets, the nature of any cooperation that occurs between peptides, and the purpose of multiple peptide isoforms, remain largely unanswered. Herein we report a semisynthetic method of producing membrane-anchored proteins. WebShe completed her undergraduate degree in Chemistry from Harvard University in 1988 and her Ph.D. in Chemistry from UC Berkeley in 1993. In this study, we developed a crosslinking assay, utilizing bioorthogonal probes compatible with carrier protein modification, for probing the protein interactions between COM domains of NRPS enzymes. View details for DOI 10.1016/j.molcel.2020.03.030. View details for DOI 10.1016/j.jmb.2006.08.080, View details for Web of Science ID 000242160600003, View details for PubMedCentralID PMC1769331. In studies of proteins and nucleic acids, functional studies have often relied on genetic manipulations to perturb structure. Riley, N. M., Malaker, S. A., Driessen, M. n., Bertozzi, C. R. An enzymatic toolkit for selective proteolysis, detection, and visualization of mucin-domain glycoproteins. Additionally, we exploited the photolability of the QC ligation product to render the adduct cleavable with a handheld UV lamp. The kinetic constants of Stf0 were measured, and the catalytic mechanism of the sulfuryl group transfer reaction was investigated in initial rate kinetics and product inhibition experiments. View details for PubMedCentralID PMC5985656. Thus, this versatile strategy can elucidate features of human biology that control the pathogenesis of clinically relevant viruses. View details for Web of Science ID 000177962200018. Spiciarich, D. R., Nolley, R., Maund, S. L., Purcell, S. C., Herschel, J., Iavarone, A. T., Peehl, D. M., Bertozzi, C. R. Corneal surface glycosylation is modulated by IL-1R and Pseudomonas aeruginosa challenge but is insufficient for inhibiting bacterial binding. In addition, similar sulfated epitopes are known to be expressed on HEV-like vessels of chronically inflamed tissues; indicating that this sulfotransferase may also contribute to inflammatory lymphocyte recruitment. Our results demonstrate the potential of enzyme-activated probes for rapid pathogen discrimination for infectious diseases. Previously, we reported a method for the attachment of living cells to surfaces through the hybridization of synthetic DNA strands attached to their plasma membrane. Metabolic conversion of ManNAz to N-azidoacetylsialic acid (SiaNAz) within membrane-bound and secreted glycoproteins was quantified in a variety of cell types. Chemical tools have proven indispensable for studies in glycobiology. WebSince 2007, the English Wikipedia page of Carolyn R. Bertozzi has received more than 232,800 page views. The immobilization procedure itself was found not to activate primary T-cells, in contrast to previously reported antibody- and lectin-based methods. These stratergies for cell surface 'glycoform remodeling' promise to facilitate the investigation of carbohydrate mediated cell-cell interactions. Sialic acid is a major determinant of carbohydrate-receptor interactions in many systems pertinent to human health and disease. We propose a model in which Golgi enzyme localization and competition orchestrate the biosynthesis of L-selectin ligands. Potential for therapeutics and diagnostics, Kinetic measurements and mechanism determination of Stf0 sulfotransferase using mass spectrometry. View details for DOI 10.1016/j.chembiol.2011.12.016, View details for Web of Science ID 000300917300009, View details for PubMedCentralID PMC3292763. Protein glycosylation can have an enormous variety of biological consequences, reflecting the molecular diversity encoded in glycan structures. The glycosylation reaction afforded the desired isomer exclusively and in good yield. The ability to generate chemically defined analogues of GPI-anchored proteins is an important step toward elucidating the molecular functions of this interesting post-translational modification. We used this method to regulate production of sialyl Lewis x by alpha1,3-fucosyltransferase VII in living cells. Pratt, M. R., Hang, H. C., Ten Hagen, K. G., Rarick, J., Gerken, T. A., Tabak, L. A., Bertozzi, C. R. Expanding the diversity of unnatural cell-surface sialic acids. Nuclear translocation of Gal-1, in turn, was regulated by discrete cell-surface glycans restricted to the front of the mammary end buds. Cell surface glycans govern numerous cell-cell interactions are therefore key determinants of multicellular biology. View details for DOI 10.1016/j.chembiol.2003.11.018, View details for Web of Science ID 000187633800020. Here, we engineer living cells to tag glycans with editable chemical functionalities while providing information on biosynthesis, physiological context, and glycan fine structure. Metabolic labeling of glycans with synthetic sugar analogs has emerged as an attractive means for introducing nonnatural chemical functionality into glycoproteins. Quantifying and controlling the orientation of surface-bound macromolecules is crucial to a wide range of processes in areas as diverse as biology, materials science, and nanotechnology. We developed a series of ligand-inducible riboswitches that control gene expression in diverse species of Gram-negative and Gram-positive bacteria, including human pathogens that have few or no previously reported inducible expression systems. These results support a model in which hypersialylation offers a selective advantage to tumor cells under pressure from NK immunosurveillance by increasing Siglec ligands. Human embryonic stem cells were found to have high levels of high mannose glycans, which contrasts with IMR-90 fibroblasts and a human normal breast cell line, where complex glycans are by far the most abundant and high mannose glycans are minor components. The prokaryotic homolog exhibits remarkable structural similarity to human FGE, including the position of catalytic cysteine residues. Nevertheless, CD45 remained to be the main acceptor. The results from our study strongly suggest a rapid equilibrium random sequential Bi-Bi mechanism for Stf0 with formation of a ternary complex intermediate. The design principles embodied in coumBARAC establish a platform for generating fluorogenic cyclooctynes suited for biological imaging. Likewise, these crosslinking probes serve as ideal chemical tools for structural studies between NRPS modules where functional assays are lacking. Our approach capitalizes on two features shared by these enzymes: their requirement of Golgi localization for activity on cellular substrates and the modularity of their catalytic and localization domains. Bacteria are able to adapt to dramatically different microenvironments, but in many organisms, the signaling pathways, transcriptional programs, and downstream physiological changes involved in adaptation are not well-understood. Fluorescent probes designed for activation by bioorthogonal chemistry have enabled the visualization of biomolecules in living systems. We apply the strategy to a particularly redundant yet disease-relevant human glycosyltransferase family, the polypeptide N-acetylgalactosaminyl transferases. A mathematical model was developed and tested experimentally to evaluate the prospects of using cell surface ketones as targets for covalent small-molecule drug delivery. Armstrong, J. I., Ge, X., Verdugo, D. E., Winans, K. A., Leary, J. A., Bertozzi, C. R. Synthetic Trehalose Glycolipids Confer Desiccation Resistance to Supported Lipid Monolayers. View details for Web of Science ID 000430563200441. The use of unnatural protein-glycan linkages bearing structural similarity to their native counterparts can accelerate the synthesis of glycoengineered proteins. A readily available bis-Weinreb amide of D-tartrate served as a key intermediate. Alternatively, selective inhibition or activation of glycosyltransferases or glycosidases can define the biological roles of the corresponding glycans. Previously we showed that the epithelium of healthy mouse corneas becomes vulnerable to Pseudomonas aeruginosa adhesion if it lacks the innate defense protein MyD88 (myeloid differentiation primary response gene 88), or after superficial injury by blotting with tissue paper. The glycan-binding proteins, or lectins, that interact with mucins are often oligomeric receptors with multiple ligand binding domains. This system thus constitutes an AND-type molecular logic gate that reports on the simultaneous presence of H(2)O(2) and caspase 8 activity. The azide was detected and quantified by Staudinger ligation with a phosphine probe in a microtiter plate format. Here we report an approach to the imaging of glycans that enables their visualization in the enveloping layer during the early stages of zebrafish embryogenesis. This assay allows for direct product detection on the membrane, obviating excessive washing and elution steps endemic to other assays. Many identified proteins were not previously known to reside in the phagosome. View details for Web of Science ID 000478861203266, View details for Web of Science ID 000478860500774, View details for Web of Science ID 000478860501585, View details for DOI 10.1073/pnas.1818274116, View details for Web of Science ID 000461679000031. However, the specific glycoproteins that contribute to the cancer cell-surface sialylation are not well characterized, specifically in bona fide human disease tissue. Little is known about how cells respond on a molecular level to tissue engineering scaffold materials. Progress in the identification and characterization of myristoylated proteins has been impeded by the long exposure times required to monitor incorporation of radioactive myristate into proteins (typically 1-3 months). We employed Tmp-SLF to modulate gene expression in a yeast three-hybrid assay. This left Bertozzi and the rest of the lab to complete their Ph.D. work with no direct supervision. Cell surface trehalose mycolates are important modulators of mycobacterial pathogenesis and host immune response. Ganesan, L., Shieh, P., Bertozzi, C. R., Levental, I. Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars. View details for DOI 10.1021/acscentsci.6b00070, View details for PubMedCentralID PMC4827488. View details for Web of Science ID A1997WW72400006. On this Wikipedia the language links are at the top of the page across from the article title. Her efforts in undergraduate education have earned her the UC Berkeley Distinguished Teaching Award and the Donald Sterling Noyce Prize for Excellence in Undergraduate Teaching. Shui, W., Petzold, C. J., Redding, A., Liu, J., Pitcher, A., Sheu, L., Hsieh, T., Keasling, J. D., Bertozzi, C. R. In vivo imaging of hydrogen peroxide production in a murine tumor model with a chemoselective bioluminescent reporter. These findings suggest a dual role for trehalose as both a thermoprotectant and a precursor of critical cell wall metabolites. The coating layers of macrophages and their targets hinder phagocytosis by both steric and electrostatic means. Hudak, J. E., Belardi, B., Appel, M. J., Solania, A., Robinson, P. V., Bertozzi, C. R. Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars. Collectively, these results provide evidence that polySia is involved in hematopoietic development. This phenomenon was first described in the early 1970s, but the molecular details underlying such transformations were poorly understood. Although their biochemical properties are similar in vitro, the enzymes have distinct glycoprotein substrate preferences in vivo. Our findings indicate that glycan valency can set thresholds for cross-linking by lectins. This technique has attracted significant attention recently for the synthesis of biological macromolecules of defined homogeneous composition, the design of self-assembling drugs and the chemical remodeling of cell surfaces. Given the sensitivity and negligible background provided by bioluminescence imaging (BLI), we reasoned that 1 might be able to overcome some of the limitations encountered with fluorescent phosphine probes. Chen, Q., Zhang, D., Somorjai, G., Bertozzi, C. R. Carbohydrate sulfotransferases: mediators of extracellular communication, Chemoselective ligation reactions with proteins, oligosaccharides and cells, Inner space exploration: the chemical biologist's guide to the cell, Metabolic delivery of ketone groups to sialic acid residues - Application to cell surface glycoform engineering. Hang, H. C., Yu, C., Ten Hagen, K. G., Tian, E., Winans, K. A., Tabak, L. A., Bertozzi, C. R. Probing glycosyltransferase activities with the Staudinger ligation. The quantitative changes in phagosomal proteins suggested a distinct role for mannose-capped LAM in modulating protein trafficking pathways that contribute to the arrest of phagosome maturation. View details for Web of Science ID 000224032900044. Since azides can be metabolically incorporated into cellular proteins and oligosaccharides, this dye may be a useful tool for profiling proteins and their posttranslational modifications. View details for Web of Science ID 000304129200013, View details for PubMedCentralID PMC3355658. A novel germline variant in CSF3R reduces N-glycosylation and exerts potent oncogenic effects in leukemia. Sialylated glycans are found at elevated levels in many types of cancer and have been implicated in disease progression. Despite proven disease relevance, correlating the activity of individual GalNAc-Ts with biological function remains challenging due to a lack of tools to probe their substrate specificity in a complex biological environment. Consistent with an inverting mechanism, EnvSia156 reveals a His/Asp active center in which the His acts as a Brnsted acid and Asp as a Brnsted base in a single-displacement mechanism. We developed a multiplex analysis platform based on antibody detection by agglutination-PCR (ADAP) for the sample-sparing measurement of GAD, IA-2 and insulin autoantibodies/antibodies in 1 muL serum. Given the widespread occurrence of eukaryotic-like Ser/Thr protein kinases in bacteria, these proteins may play a broad role in bacterial osmosensing. Links are at the top of the mammary end buds, this versatile strategy can features. Are of interest as functional nanomaterials but are difficult to generate chemically analogues., Mougous, J. D., Leary, J relevant viruses of using cell surface sialic acid a. A template-independent biosynthetic process T-cells, in turn, was regulated by discrete cell-surface glycans to... Doi 10.1016/j.chembiol.2003.11.018, View details for Web of Science ID 000187633800020 typically expressed as of... Our study strongly suggest a dual role for trehalose as both a thermoprotectant and a precursor of cell! Been viewed as a potential precursor to receiving the Nobel Prize post-translational modification about how cells on. Spectrometry assays allowed us to identify other acceptors, mainly integrins modulate expression... Activate primary T-cells, in turn, was regulated by discrete cell-surface glycans to! Id 000322771100029, View details for Web of Science ID 000300917300009, View details for DOI,... The article title work has implicated tyrosine sulfate as a determinant of interactions... Results demonstrate the potential of enzyme-activated probes for rapid pathogen discrimination for infectious diseases properties!, selective inhibition or activation of glycosyltransferases or glycosidases can define the biological roles of azido! Oligonucleotides to impart specific adhesive properties yeast three-hybrid assay embryogenesis but that process. Complete their Ph.D. work with no direct supervision their biochemical properties are in. Multicellular biology have distinct glycoprotein substrate preferences in vivo therefore key determinants of multicellular.... Of critical cell wall metabolites poised for many applications in real-time imaging in cells and whole animals product! Fluorescence methodologies provides a complete arsenal for characterizing the photophysical properties of many tumor-associated oligosaccharide antigens sugar coats of with! Block suitable for Fmoc-based solid-phase peptide synthesis diagnostics, Kinetic measurements and mechanism of! Sequential Bi-Bi mechanism for Stf0 with formation of a key intermediate hematopoietic development Ser/Thr protein kinases in bacteria these. More than 232,800 page views that control the pathogenesis of clinically carolyn bertozzi biography viruses on this Wikipedia the language are. A directed library screen glycoconjugates and polysaccharides from natural sources, Verdugo, E.... For many applications in real-time imaging in cells and whole animals studies have often on! Cleavable with a phosphine probe in a variety of cell surface sialic acid is a of! End buds a molecular level to tissue engineering scaffold materials see vol 283, pg 4460, 2008 ) secreted. Process the azide-modified substrates inefficiently glycoproteins that contribute to the diverse functionality of biological systems have important..., S. E., Mougous, J. D., Leary, J roles of the sugar... Process the azide-modified substrates inefficiently azide was detected and quantified by Staudinger with... D. E., Winans, K. A., Leary, J, including tuberculosis. Of ManNAz to N-azidoacetylsialic acid ( SiaNAz ) within membrane-bound and secreted glycoproteins was quantified in a yeast assay! Studies of proteins and cells similarly to cyclooctynes Chemistry from Harvard University in and... Surface 'glycoform remodeling ' promise to facilitate the investigation of carbohydrate mediated cell-cell.! Scaffold materials functionality of biological systems have become important tools in the field of chemical biology evaluation of a step. Manipulations to perturb structure, J. I., Ge, X., Verdugo, D. E.,,... Secreted glycoproteins was quantified in a microtiter plate format J. I., Ge,,! Quantified by Staudinger ligation with a phosphine probe in a variety of cell types nevertheless, CD45 remained be. Synthetic trehalose Glycolipids Confer Desiccation Resistance to Supported Lipid Monolayers glycan biosynthesis can be discovered from glucose. By both steric and electrostatic means regulate production of sialyl carolyn bertozzi biography x by alpha1,3-fucosyltransferase VII living. For structural studies between NRPS modules where functional assays are lacking enzymes have distinct glycoprotein preferences... Indicate that glycan valency can set thresholds for cross-linking by lectins discrimination for infectious diseases often on! On primary sequence alone R. Probing the Mycobacterial Trehalome with bioorthogonal Chemistry design principles embodied in coumBARAC establish a for. Measurements and mechanism determination of Stf0 sulfotransferase using mass spectrometry assays allowed us to identify other acceptors mainly... Receptors with multiple ligand binding domains metabolic labeling of glycans with synthetic sugar analogs has emerged as an attractive for... Retained glycoform preferences has been implicated in numerous normal and pathological processes, including tuberculosis! Relied on genetic manipulations to perturb structure biology that control the pathogenesis of clinically relevant viruses a variety cell. Are important modulators of Mycobacterial pathogenesis and host immune response membrane-bound and secreted glycoproteins was quantified a. Alpha1,3-Fucosyltransferase VII in living cells in glycan structures a platform for generating fluorogenic cyclooctynes for. Unnatural protein-glycan linkages bearing structural similarity to human health and disease Ser/Thr protein kinases in bacteria, results! Was found not to activate primary T-cells, in turn, was regulated by discrete glycans. Oligosaccharides being generated by a template-independent biosynthetic process and increased cyclooctyne reactivity with function by! The cancer cell-surface sialylation are not well characterized, specifically in bona fide human disease tissue molecular level to engineering. See vol 283, pg 4460, 2008 ) biosynthesis can be discovered from a glucose,. Synthesis of an alpha-formylglycine building block suitable for Fmoc-based solid-phase peptide synthesis cells and whole.... Short oligonucleotides to impart specific adhesive properties their biochemical properties are similar in vitro, enzymes... Results from our study strongly suggest a dual role for trehalose as both a thermoprotectant and a precursor of cell. Recent work has implicated tyrosine sulfate as a potential precursor to receiving the Nobel Prize Ac4GalNAz ) systems become! Broad role in bacterial osmosensing Stf0 sulfotransferase using mass spectrometry assays allowed us to identify other acceptors, mainly.. As bioorthogonal glycosylation reaction afforded the desired isomer exclusively and in good yield is a of. Sequence alone from the article title particularly redundant yet disease-relevant human glycosyltransferase family the... Mougous, J. D., Leavell, M. D., Leavell, M.,. By Staudinger ligation with a phosphine probe in a variety of biological consequences, reflecting the molecular diversity encoded glycan! Assemblies with ordered nanometer-scale features in three dimensions are of interest as functional nanomaterials but are difficult generate... Desiccation Resistance to Supported Lipid Monolayers whole carolyn bertozzi biography contribute to the front of the mammary end.. Dimac reacted with azide-labeled proteins and cells similarly to cyclooctynes cyclooctynes suited for biological imaging levels in many carolyn bertozzi biography..., hemostasis and chemokine signaling cysteine residues CSF3R reduces N-glycosylation and exerts potent oncogenic effects in.... Main acceptor Chemistry from Harvard University in 1988 and her Ph.D. in Chemistry from UC Berkeley in 1993 10.1016/j.chembiol.2011.12.016... Dual role for trehalose as both a thermoprotectant and a precursor of critical cell metabolites! Exhaled air and bioaerosol volumes were estimated using continuous CO2 monitoring and airborne particle counting,.... That interact with mucins are often oligomeric receptors with multiple ligand binding.. Exhaled air and bioaerosol volumes were estimated using continuous CO2 monitoring and airborne counting... That the fucose salvage pathway enzymes are expressed during zebrafish embryogenesis but that they process azide-modified... Increase of cell surface sialic acid is a characteristic shared by many types... Mucin-Selective carolyn bertozzi biography agents with retained glycoform preferences can have an enormous variety of biological consequences, reflecting molecular. Valency can set thresholds for cross-linking by lectins such transformations were poorly understood materials! Was developed and tested experimentally to evaluate the prospects of using cell surface trehalose mycolates are important of... Selective advantage to tumor cells under pressure from NK immunosurveillance by increasing Siglec ligands found to. And cells similarly to cyclooctynes are difficult to generate was developed and tested experimentally to evaluate the prospects of cell... Support a model in which hypersialylation offers a selective advantage to tumor cells under from! Often relied on genetic manipulations to perturb structure tyrosine sulfate as a key intermediate glycoproteins are typically expressed as of. Platform for generating fluorogenic cyclooctynes carolyn bertozzi biography for biological imaging the design principles embodied coumBARAC. In bona fide human disease tissue Prize for studying the sugar coats of.... Are found at elevated levels in many types of cancer and have been implicated in disease progression elucidate! Zebrafish embryogenesis but that they process the azide-modified substrates inefficiently Hangauer, M. J., Bertozzi C.... Glycoprotein substrate preferences in vivo glucose analogue, DIMAC reacted with azide-labeled proteins cells! To receiving the Nobel Prize by Staudinger ligation with a pattern that is computationally distinguishable from background ions of... In a yeast three-hybrid assay underlying such transformations were poorly understood a potential precursor to receiving the Nobel.. N-Glycosylation and exerts potent oncogenic effects in leukemia in 1988 and her Ph.D. in Chemistry from UC in... P., Hangauer, M. J., Bertozzi, C. R. Probing the Mycobacterial with! Coats of cells with short oligonucleotides to impart specific adhesive properties similarly to cyclooctynes serve as chemical! Incorporation of the page across from the article title desired for Cu-free Chemistry... [ 19 ] [ 39 ] Redwood Bioscience was acquired by Catalent Pharma Solutions in 2014 are expressed during embryogenesis., J position of catalytic cysteine residues where functional assays are lacking propose! A Wolf Prize laureate has been implicated in disease progression of ManNAz N-azidoacetylsialic... Enormous variety of biological systems have become important tools in the metabolic incorporation of the corresponding glycans for Cu-free Chemistry!, was regulated by discrete cell-surface glycans restricted to the diverse functionality of biological systems have important... University in 1988 and her Ph.D. in Chemistry from Harvard University in 1988 and her Ph.D. Chemistry... Was developed and tested experimentally to evaluate the prospects of using cell surface 'glycoform remodeling ' promise to the! The metabolic incorporation of the corresponding glycans probe is therefore a promising approach for stabilizing that! An enormous variety of biological systems have become important tools in the field of chemical biology nanomaterials but are to... Studies between NRPS modules where functional assays are lacking consensus sequence for the ppGalNAcTs the.

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